关键词: 门静脉血栓, 大鼠模型, 血栓机化, 血流淤滞

Abstract: Objective This study aimed to construct a stable portal vein thrombosis ( PVT) model based on different factors of thrombosis, and to observe the organized evolution of PVT dynamically. Method One hundred and twenty SD rats were randomly divided into three groups. In group A, the blood flow was interrupted by microvascular clamp, which caused the blood stasis in the portal vein. In group B, only anhydrous alcohol was used to destroy vascular intima. Group C combined the above two methods to prepare PVT. The formation of PVT after the modeling was observed by macroscopic observation and vascular ultrasound. The evolution of thrombus at 1, 3, 5 and 7 days after the modeling was observed by H&E staining dynamically. Result There was no obvious thrombosis in group A with simple blood stasis. Compared with group B with simple endothelial injury, group C with blood stasis combined with endothelial injury could form more stable PVT, and half of the thrombus remained at 7 days after surgery. H&E staining revealed the gradual organization of the thrombus over time, and the thrombus was basically recanalized 5 to 7 days after surgery. Some of the portal vein wall fibrosis and thickening lesions were observed and extended into the portal vein lumen. Conclusion The method of interrupting the portal blood flow and destroying the portal vein intima by anhydrous alcohol can be used to construct the PVT model in rats stably, simply and in line with clinical characteristics. During the process of thrombosis from formation to organization and recanalization, some portal vein walls thickened and became fibrosis, resulting in partial portal vein stenosis. 

Key words: portal vein thrombosis, rat model, thrombus organization, blood stasis